Device

Part:BBa_K2088672:Design

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-09)


copA + riboJ +mhbDHIM + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2518
    Illegal BamHI site found at 2904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal NgoMIV site found at 754
    Illegal NgoMIV site found at 2708
    Illegal NgoMIV site found at 3845
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3038


Design Notes

Used riboJ to help expose the rbs.


Source

This is a composite part including gene cluster(mhbDHIM) encoding the enzymes involved in the catabolism of 3-HBA to fumarate and pyruvate.

References